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Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
Published on: May 17, 2019
Bcl-2 gene expression in human breast cancers in iran
Leila Rostamizadeh1, Ashraf Fakhrjou, Vahid Montazeri
1Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Asian Pacific Journal of Cancer Prevention : APJCP
|September 3, 2013
Summary
Bcl-2 gene expression is higher in breast tumor tissues compared to non-tumor tissues. This gene expression analysis may aid in breast cancer screening and diagnosis.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Breast cancer is a leading cancer in Iranian women, often diagnosed at advanced stages.
- Early detection and molecular screening are crucial for effective breast cancer treatment.
- Altered gene expression, particularly of the Bcl-2 gene, is implicated in breast cancer development.
Purpose of the Study:
- To investigate Bcl-2 gene expression levels in paired breast tumor and non-tumor tissues.
- To assess the correlation between Bcl-2 expression and clinicopathological features of breast cancer.
Main Methods:
- RNA extraction and polyadenylation-based reverse transcription polymerase chain reaction (RT-PCR) were performed.
- Bcl-2 gene expression was quantified using real-time PCR in 40 breast tissue samples (21 tumor, 16 non-tumor, 3 benign).
Main Results:
- Bcl-2 gene expression was higher in tumor samples than in marginal non-tumor samples, though not statistically significant (p>0.05).
- Positive Bcl-2 expression was observed in 66.7% of tumor samples and 56.3% of marginal samples.
- Higher Bcl-2 expression showed a trend towards association with higher histological grade (p<0.41).
Conclusions:
- Dysregulated Bcl-2 gene expression may play a role in breast cancer pathogenesis.
- Gene expression analysis, including Bcl-2, can enhance cancer screening, diagnosis, and prognostic evaluation.
- Bcl-2 expression analysis could support clinical trials for Bcl-2-targeted therapies.
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