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High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
Kinetics of enzymatic degradation of high molecular weight polysaccharides through a nanopore: experiments and
Aziz Fennouri1, Régis Daniel, Manuela Pastoriza-Gallego
1Université d'Évry Val d'Essonne , Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement, F-91025 Evry, France.
Analytical Chemistry
|September 3, 2013
Abstract:
The enzymatic degradation of long polysaccharide chains is monitored by nanopore detection. It follows a Michaelis-Menten mechanism. We measure the corresponding kinetic constants at the single molecule level. The simulation results of the degradation process allowed one to account for the oligosaccharide size distribution detected by a nanopore.

