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Enzyme-Linked Immunosorbent Assay

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Updated: May 8, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Thrombin detection using a piezoelectric aptamer-linked immunosorbent assay.

Cheryl M Collins¹, Samuel Yui, Charles E S Roberts

¹Loxbridge Research, Royal Institution of Great Britain, London W1S 4BS, UK.

Analytical Biochemistry

|September 3, 2013

Summary

This study introduces a new aptamer-based diagnostic assay using Acoustic Membrane MicroParticle (AMMP) detection. This method offers sensitive and rapid detection of thrombin in serum, overcoming limitations of current diagnostic platforms.

Keywords:

Acoustic Membrane MicroParticle (AMMP)Aptamer Detection Diagnostic SELEX Thrombin ViBE

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Area of Science:

Biotechnology
Biosensor Technology
Molecular Diagnostics

Background:

Current diagnostic assays often rely on antibody-based capture agents, limiting specificity and efficiency.
Existing detection platforms face challenges with interfering substances in biological samples like serum.
Aptamers offer a promising alternative to antibodies as capture and detection agents due to their high specificity.

Purpose of the Study:

To demonstrate the utility of Acoustic Membrane MicroParticle (AMMP) detection for aptamer-based assays.
To develop a sensitive, reproducible, and rapid method for detecting specific targets in complex biological matrices.
To adapt the AMMP platform for clinical applications using aptamers against a relevant target.

Main Methods:

Utilized BioScale's novel frequency-modulating Acoustic Membrane MicroParticle (AMMP) detection technology.
Incorporated aptamers as both capture and detection agents within the AMMP platform.
Tested the assay's performance using thrombin detection in human serum.

Main Results:

Achieved sensitive and reproducible detection of thrombin in human serum.
Demonstrated a robust and rapid assay, bypassing labor-intensive Western blot analysis.
Showed that the AMMP platform is unaffected by common interfering substances in serum that impact optical methods.
Successfully adapted the AMMP platform for aptamer-based detection of a clinically relevant target.

Conclusions:

The AMMP platform provides a sensitive and reproducible method for aptamer-based diagnostics.
This novel approach offers a rapid and robust alternative to existing diagnostic methods, particularly in complex samples like serum.
The AMMP platform is well-suited for the development of commercial aptamer-based diagnostic assays for clinical settings.