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Updated: May 8, 2026

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)
Published on: June 20, 2016
TALENoffer: genome-wide TALEN off-target prediction
Jan Grau1, Jens Boch, Stefan Posch
1Institute of Computer Science and Department of Genetics, Institute of Biology, Martin Luther University Halle-Wittenberg, D-06099 Halle (Saale), Germany.
Summary:
Transcription activator-like effector nucleases (TALENs) have become an accepted tool for targeted mutagenesis, but undesired off-targets remain an important issue. We present TALENoffer, a novel tool for the genome-wide prediction of TALEN off-targets. We show that TALENoffer successfully predicts known off-targets of engineered TALENs and yields a competitive runtime, scanning complete mammalian genomes within a few minutes.
Availability:
TALENoffer is available as a command line program from http://www.jstacs.de/index.php/TALENoffer and as a Galaxy server at http://galaxy.informatik.uni-halle.de.
Contact:
grau@informatik.uni-halle.de
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