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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: May 8, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Practical innovations for high-throughput amplicon sequencing.

Derek S Lundberg1, Scott Yourstone, Piotr Mieczkowski

  • 11] Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA. [2] Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, USA. [3].

Nature Methods
|September 3, 2013
PubMed
Summary
This summary is machine-generated.

New methods improve 16S ribosomal RNA (rRNA) amplicon sequencing for metagenomics. Unique tagging and PCR clamps correct errors and bias, enhancing microbial sequence accuracy.

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Last Updated: May 8, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

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Published on: August 29, 2014

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

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Published on: August 29, 2025

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
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Published on: October 16, 2018

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • 16S ribosomal RNA (rRNA) amplicon sequencing is crucial for metagenomics.
  • Existing methods suffer from amplification bias and sequencing errors.
  • Contamination from eukaryotic hosts can skew microbial community analysis.

Purpose of the Study:

  • To present improved techniques for 16S rRNA amplicon sequencing.
  • To address and correct amplification bias and sequencing errors.
  • To reduce eukaryotic contamination and enrich microbial sequences.

Main Methods:

  • Unique tagging of template DNA molecules prior to PCR amplification.
  • Development of software to map amplicon sequences to original templates.
  • Application of PCR clamps to inhibit amplification of eukaryotic DNA.

Main Results:

  • The described methods enable accurate mapping of sequences to their original templates.
  • Amplification bias and sequencing errors are corrected using the provided software.
  • PCR clamps effectively enrich microbial sequences by blocking host DNA amplification.

Conclusions:

  • The improved 16S rRNA sequencing approach enhances accuracy in metagenomic studies.
  • This technique provides a more reliable method for analyzing microbial communities.
  • The developed tools offer significant advantages for microbiome research.