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The Ras Gene

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The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...

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The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...

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Related Experiment Video

Updated: May 8, 2026

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

RGS6 suppresses Ras-induced cellular transformation by facilitating Tip60-mediated Dnmt1 degradation and promoting

J Huang¹, A Stewart¹, B Maity¹

¹Department of Pharmacology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA.

|September 3, 2013

Summary

Regulator of G protein signaling 6 (RGS6) suppresses Ras-induced cancer by promoting degradation of DNA methyltransferase 1 (Dnmt1), inhibiting cell proliferation and promoting apoptosis. RGS6 acts as a scaffold for Dnmt1 and Tip60, facilitating Dnmt1 breakdown and blocking oncogenic transformation.

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Inducible and Reversible Dominant-negative (DN) Protein Inhibition

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Last Updated: May 8, 2026

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

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Published on: December 7, 2017

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

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Published on: March 9, 2012

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Published on: January 7, 2019

Area of Science:

Molecular Biology
Oncology
Cell Signaling

Background:

RAS proto-oncogene mutations drive cancer by promoting cell proliferation.
DNA methyltransferase 1 (Dnmt1) silences tumor suppressors, aiding Ras-driven transformation and is overexpressed in cancers.

Purpose of the Study:

To investigate the role of Regulator of G protein signaling 6 (RGS6) in suppressing Ras-induced cellular transformation.
To elucidate the mechanism by which RGS6 regulates Dnmt1 expression and function.

Main Methods:

Utilized mouse embryonic fibroblasts from wild-type and RGS6 knockout mice.
Analyzed protein expression, degradation, and interactions using biochemical assays.
Investigated the role of RGS6 as a scaffolding protein in the Dnmt1-Tip60 complex.

Main Results:

Oncogenic Ras upregulated RGS6, which blocked Ras-induced cellular transformation.
RGS6 facilitated Tip60-mediated acetylation, ubiquitylation, and degradation of Dnmt1.
RGS6 scaffolding activity was essential for Dnmt1 downregulation and inhibition of its anti-apoptotic function.

Conclusions:

RGS6 acts as a novel tumor suppressor by targeting Dnmt1 for degradation, thereby counteracting Ras-induced oncogenic transformation.
RGS6's RGS domain mediates interaction with Tip60, crucial for Dnmt1 regulation.
RGS6 represents a potential therapeutic target for cancers driven by Ras and characterized by Dnmt1 overexpression.