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Updated: May 8, 2026
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A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Published on: March 5, 2018
A rapid screen for molecules that form duplex to duplex crosslinks in DNA
María J Marín1, Benjamin D Rackham, Andrew N Round
1School of Chemistry, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK. d.russell@uea.ac.uk.
Chemical Communications (Cambridge, England)
|September 3, 2013
Summary
A new gold nanoparticle assay quickly measures DNA duplex crosslinking by ligands. This method aids in developing compounds for targeting compacted DNA, like that in nucleosomes.
Area of Science:
- Biochemistry
- Nanotechnology
- Molecular Biology
Background:
- Ligand-induced DNA crosslinking is crucial for understanding DNA structure and function.
- Targeting compacted DNA, such as nucleosomal DNA, presents therapeutic challenges.
Purpose of the Study:
- To develop a rapid and sensitive assay for quantifying DNA duplex crosslinking by ligands.
- To explore the potential of this assay in studying interactions with compacted DNA structures.
Main Methods:
- Utilized gold nanoparticles to create a novel assay.
- Developed a method to detect and quantify the crosslinking of DNA duplexes induced by various ligands.
- Applied the assay to investigate ligand interactions with DNA.
Main Results:
- The gold nanoparticle assay demonstrated rapid and accurate determination of DNA crosslinking.
- Successfully quantified the crosslinking efficiency of different ligands.
- Validated the assay's applicability for studying DNA-ligand interactions.
Conclusions:
- The developed gold nanoparticle assay provides a powerful tool for studying DNA crosslinking.
- This assay has significant potential for drug discovery and understanding DNA-protein interactions.
- Facilitates research into targeting compacted DNA structures for therapeutic interventions.
