Related Experiment Video
Updated: May 8, 2026

12:20
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic
Joon Soo Park1, Ahruem Baek, Il-Soo Park
1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. kimde@konkuk.ac.kr.
Abstract:
We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface.

