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Related Concept Videos

Data Collection by Experiments01:13

Data Collection by Experiments

Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.”
An example of the experimental method is a public clinical trial...
Statistical Methods for Analyzing Epidemiological Data01:25

Statistical Methods for Analyzing Epidemiological Data

Epidemiological data primarily involves information on specific populations' occurrence, distribution, and determinants of health and diseases. This data is crucial for understanding disease patterns and impacts, aiding public health decision-making and disease prevention strategies. The analysis of epidemiological data employs various statistical methods to interpret health-related data effectively. Here are some commonly used methods:
Mechanistic Models: Compartment Models in Individual and Population Analysis01:23

Mechanistic Models: Compartment Models in Individual and Population Analysis

Mechanistic models are utilized in individual analysis using single-source data, but imperfections arise due to data collection errors, preventing perfect prediction of observed data. The mathematical equation involves known values (Xi), observed concentrations (Ci), measurement errors (εi), model parameters (ϕj), and the related function (ƒi) for i number of values. Different least-squares metrics quantify differences between predicted and observed values. The ordinary least squares (OLS)...
Investigation of Disease Outbreaks01:23

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: May 8, 2026

In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

The reverse-direction method links mass experimental data to human diseases.

Hideki Ogura1, Toru Atsumi, Hidenori Bando

  • 1Laboratory of Developmental Immunology JST-CREST, Graduate School of Frontier Biosciences, Graduate School of Medicine, and WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.

Archivum Immunologiae Et Therapiae Experimentalis
|September 3, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel reverse-direction method to link large-scale genetic screening data to human diseases. This approach identified the epiregulin pathway as a key regulator of inflammation, offering new insights into chronic inflammatory disorders.

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In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
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In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Published on: August 24, 2013

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
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In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Area of Science:

  • Genomics
  • Immunology
  • Molecular Biology

Background:

  • Genome-wide screenings generate vast datasets for biological process elucidation.
  • Extrapolating such data to human diseases remains a critical challenge for researchers.
  • The inflammation amplifier, a NFκB/STAT3-dependent pathway, drives immune cell recruitment and inflammation.

Purpose of the Study:

  • To elucidate the molecular mechanisms of the inflammation amplifier.
  • To develop a method for linking high-throughput screening data to human diseases.
  • To identify novel regulators of inflammation associated with human pathologies.

Main Methods:

  • Functional genome-wide screenings were performed to study the inflammation amplifier.
  • A reverse-direction method utilizing public genome-wide association studies (GWAS) databases was employed.
  • Data analysis focused on linking screening results to human disease associations.

Main Results:

  • The epiregulin-epidermal growth factor receptor pathway was identified as a regulator of the inflammation amplifier.
  • This pathway was successfully linked to human diseases via GWAS.
  • Elevated serum epiregulin levels were observed in patients with chronic inflammatory disorders.

Conclusions:

  • The reverse-direction method effectively links mass screening data to human disease-related genes.
  • This approach facilitates the identification of disease-associated molecular mechanisms.
  • The epiregulin pathway represents a potential therapeutic target for chronic inflammatory conditions.