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Enhanced lycopene content in Blakeslea trispora by effective mutation-screening method.

Qiang Wang¹, Wei Luo, Qiu-Ya Gu

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Applied Biochemistry and Biotechnology

|September 3, 2013

Summary

N(+) ion implantation enhanced lycopene production in Blakeslea trispora. This method yielded a mutant with 64% higher lycopene than the parent strain, proving more effective than NTG treatment.

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Area of Science:

Mycology
Biotechnology
Biochemistry

Background:

Blakeslea trispora is a zygomycete fungus utilized for natural production of carotenoids like lycopene and beta-carotene.
Optimization of lycopene yield from B. trispora is crucial for industrial applications.

Purpose of the Study:

To enhance lycopene and biomass yield in Blakeslea trispora.
To compare the efficacy of N(+) ion implantation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis for strain improvement.

Main Methods:

Mutagenesis of B. trispora (-) strain using N(+) ion implantation and NTG.
Screening of mutants on media supplemented with lovastatin and crude trisporic acid extracts (CTA).
Quantification of lycopene and biomass yield in isolated mutants.

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Main Results:

Four mutants with increased lycopene and biomass yield were isolated.
A mutant (I5) generated via N(+) ion implantation exhibited a maximum lycopene yield of 28.8 mg/g, a 64% increase over the parent strain.
N(+) ion implantation proved more effective than NTG for B. trispora mutagenesis in this study.

Conclusions:

N(+) ion implantation is a suitable method for improving lycopene production in Blakeslea trispora.
Lovastatin aids in the selection of beneficial mutants, while CTA enhances visual screening.
The study identified a superior mutant for enhanced carotenoid production.