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Updated: May 8, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

self-assembling GFP: a versatile tool for plant (membrane) protein analyses.

Katharina Wiesemann¹, Lucia E Groß, Manuel Sommer

¹Cluster of Excellence Frankfurt, Center for Membrane Proteomics, Department of Biosciences, Molecular Cell Biology, Goethe University, Frankfurt, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|September 3, 2013

Summary

This summary is machine-generated.

Analyzing plant cell molecular networks is key. Self-assembling GFP enables in vivo membrane protein analysis, detailing protein distribution and topology in Arabidopsis thaliana protoplasts.

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Area of Science:

Plant molecular biology
Cellular and subcellular processes

Background:

Understanding plant cell molecular networks is crucial for deciphering cellular functions.
Self-assembling fluorescent proteins (SA-GFP) offer a powerful method for analyzing proteins, particularly membrane proteins.
SA-GFP relies on the autocatalytic reassembly of nonfluorescent strands (1-10 and 11) to generate fluorescence.

Purpose of the Study:

To establish protocols for analyzing membrane protein topology in vivo.
To demonstrate the utility of self-assembling GFP for studying protein distribution in plant cells.

Main Methods:

Utilizing self-assembling GFP (SA-GFP) technology.
Analyzing protein distribution and membrane protein topology.
Employing Arabidopsis thaliana protoplasts as the experimental system.
Developing basic protocols for in vivo analysis.

Main Results:

Demonstrated the feasibility of using SA-GFP to analyze membrane protein topology in plant protoplasts.
Provided foundational protocols for researchers to apply this technique.
Enabled visualization of protein distribution within the cellular membrane.

Conclusions:

Self-assembling GFP is a versatile tool for in vivo membrane protein analysis in plants.
The developed protocols facilitate the study of membrane protein topology and distribution.
This technique enhances our understanding of molecular mechanisms in plant cells.