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Related Experiment Video

Updated: May 8, 2026

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
11:56

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

Published on: August 24, 2015

3D-tissue model for herpes simplex virus-1 infections.

Ina Hogk1, Steffen Rupp, Anke Burger-Kentischer

  • 1Institute for Interfacial Engineering and Plasma Technology IGVP, University of Stuttgart, Stuttgart, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2013
PubMed
Summary

Researchers developed a novel in vitro model for herpes simplex virus type 1 (HSV-1) infection using a human skin equivalent. This model overcomes limitations of animal studies for investigating HSV-1 latency and reactivation.

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Area of Science:

  • Virology
  • Dermatology
  • Infectious Diseases

Background:

  • Herpes simplex virus type 1 (HSV-1) causes common skin infections.
  • Studying HSV-1 latency and reactivation is crucial but challenging.
  • Current animal models have limitations, and no in vitro model accurately mimics natural latency.

Purpose of the Study:

  • To establish the first in vitro model for HSV-1 infection using a human skin equivalent.
  • To overcome the limitations of animal models for studying HSV-1 latency and reactivation.
  • To provide a more accessible and ethical platform for HSV-1 research.

Main Methods:

  • Developed a 3D human skin equivalent model.
  • Utilized the human keratinocyte cell line HaCaT.
  • Incorporated human primary fibroblasts and a quiescently HSV-1 infected neuronal component.

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Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
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Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

Published on: November 3, 2012

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Related Experiment Videos

Last Updated: May 8, 2026

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
11:56

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

Published on: August 24, 2015

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
07:55

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

Published on: November 3, 2012

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Main Results:

  • Successfully created an in vitro HSV-1 infection model.
  • The model is based on a human skin equivalent, integrating multiple human cell types.
  • This system allows for the study of HSV-1 infection, latency, and reactivation in a controlled environment.

Conclusions:

  • The developed in vitro model represents a significant advancement in studying HSV-1.
  • It offers a promising alternative to animal models for investigating viral latency and reactivation.
  • This human skin equivalent model has the potential to accelerate research into HSV-1 pathogenesis and therapeutic strategies.