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Updated: May 8, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Gene targeting in mice: a review.
Hicham Bouabe1, Klaus Okkenhaug
1Laboratory of Lymphocyte Signalling and Development, Babraham Institute, Cambridge, UK.
Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2013
Summary
Gene targeting in mice allows researchers to modify DNA sequences, creating powerful models for studying gene function and human diseases. Advanced techniques enable precise gene editing and conditional expression for in-depth biological research.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Animal Models
Background:
- Mice are essential experimental models in biological and medical research.
- Germline genome modification allows for the study of gene function and disease pathogenesis.
- Gene targeting techniques enable precise manipulation of the mouse genome.
Purpose of the Study:
- To provide an updated summary of gene targeting technologies in mice.
- To discuss key considerations in the design of gene-targeted mouse models.
- To highlight the applications of gene targeting in fundamental and medical research.
Main Methods:
- Random DNA integration into the mouse genome.
- Homologous recombination for targeted gene modification.
- Application of recombination systems (e.g., Cre/loxP, FRT/FLP) for conditional gene expression.
Main Results:
- Gene targeting enables gene deletion, insertion, and mutation to study gene function.
- Development of mouse models for human-specific genes and diseases.
- Introduction of reporter genes for monitoring gene expression in vivo and ex vivo.
- Conditional gene expression allows temporal and tissue-specific manipulation.
Conclusions:
- Gene targeting technology is crucial for advancing our understanding of genetics and disease.
- Careful design of gene-targeted mice is essential for successful research outcomes.
- The versatility of gene targeting enhances the utility of mice as experimental models.
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