The measurement of anti-Müllerian hormone (AMH)

Anti-Müllerian hormone (AMH) has long been associated with sexual differentiation in the fetus where in the male it suppresses the development of the female reproductive tracts. More recently it has been shown to be secreted in low concentrations by the granulosa cells of the adult and related to follicular development in the ovary. A number of laboratories are now measuring this hormone as part of IVF treatment and in the investigation of polycystic ovarian disease in particular. Early immunoassays for this hormone were not stable and currently laboratories are using the assay sold by Beckman Coulter, Inc., the AMH Gen II ELISA. This assay has been developed from two commercial assays purchased by Beckman and is reported to be precise and convenient. It is the assay described here.