Effect of lasalocid on the development of Eimeria tenella in chicken embryos

Sporozoites of Eimeria tenella were treated with lasalocid in vitro and their viability tested by inoculating them into the allantoic cavity of 11-day-old chicken embryos. Concentrations of 0.1, 1.0 and 10.0 micrograms ml-1 reduced sporozoite viability, as judged by oocyst production. Injections into the embryos of 5, 50 or 500 micrograms of lasalocid 92-93 h after infection also reduced oocyst production, indicating activity against the later stages of the life cycle.