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Quantitative liver lesion volume determination by nanoparticle-based SPECT.

Dániel S Veres1, Domokos Máthé, Ildikó Futó

  • 1Department of Biophysics and Radiation Biology, Semmelweis University, 1094, Budapest, Hungary.

Molecular Imaging and Biology
|September 3, 2013
PubMed
Summary

This study introduces a simple method using technetium-99m macro-aggregated albumin ([(99m)Tc]-MAA) for quantitative liver imaging. The technique effectively identifies and quantifies liver tumor burden by detecting cold spots in SPECT/CT scans.

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Area of Science:

  • Nuclear Medicine
  • Medical Imaging
  • Oncology

Background:

  • Kupffer cells play a crucial role in liver function and disease, particularly in liver cancer.
  • Accurate quantification of liver tumor burden is essential for diagnosis and treatment monitoring.
  • Existing imaging methods may lack the simplicity and quantitative precision required for liver lesion assessment.

Purpose of the Study:

  • To develop and validate a simple, quantitative data analysis method for liver single-photon emission computed tomography (SPECT) imaging.
  • To establish in vivo quantitative SPECT/computed tomography (CT) imaging protocols for determining liver tumor burden.
  • To explore the potential of radiolabeled macro-aggregated albumin particles for imaging liver disease and quantifying cold spot lesions.

Main Methods:

  • Utilized radiolabeled macro-aggregated albumin particles ([(99m)Tc]-MAA) injected intravenously to image liver disease in mice.
  • Employed a small animal SPECT/CT system for in vivo imaging of healthy controls and diethylnitrosamine-induced hepatocellular carcinoma (HCC) models.
  • Quantified liver radioactivity, segmented the liver using Otsu thresholding, and determined radioactivity volume and total liver activity.

Main Results:

  • [(99m)Tc]-MAA SPECT/CT scans revealed cold spots, indicative of tumors, in all diseased animals.
  • Quantitative analysis of volumetric maps allowed for the determination of liver tumor burden.
  • A significant difference in isotope-labeled particle uptake (standardized uptake concentration) was observed between control (median 0.33) and HCC (median 0.18) groups (p = 0.0015).

Conclusions:

  • Developed a novel application of [(99m)Tc]-MAA for quantitative liver imaging.
  • The method provides a simple and effective means to quantitatively characterize liver cold spot lesions.
  • This technique is valuable for assessing Kupffer cell dysfunction associated with tumor burden in liver cancer.