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Updated: May 8, 2026

A Simple, Low-cost, and Robust System to Measure the Volume of Hydrogen Evolved by Chemical Reactions with Aqueous Solutions

A Simple, Low-cost, and Robust System to Measure the Volume of Hydrogen Evolved by Chemical Reactions with Aqueous Solutions

Published on: August 17, 2016

Measuring hydrogen peroxide reduction using a robust, inexpensive, and sensitive method.

Ryan A Hyland¹, Peter J Rogers, Vincent J Higgins

¹School of Science and Health, University of Western Sydney, Sydney, NSW 1797 Australia.

Journal of Chemical Biology

|September 3, 2013

Summary

This summary is machine-generated.

This study introduces an improved Europium Tetracycline Hydrogen Peroxide Reduction Assay (EHRA) for measuring antioxidant activity. The EHRA method is robust, inexpensive, and effective for both pure compounds and natural extracts like hops.

Keywords:

Antioxidant assay EHRA Europium tetracycline Hops Hydrogen peroxide ROS

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Last Updated: May 8, 2026

A Simple, Low-cost, and Robust System to Measure the Volume of Hydrogen Evolved by Chemical Reactions with Aqueous Solutions

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Published on: February 24, 2018

Area of Science:

Analytical Chemistry
Biochemistry
Natural Product Analysis

Background:

Accurate measurement of antioxidant activity is crucial for various scientific disciplines.
Existing methods may have limitations in scope or cost-effectiveness.
The Europium Tetracycline assay offers a novel approach to probe-based antioxidant assessment.

Purpose of the Study:

To report an improved method for analyzing antioxidant activity using the Europium Tetracycline Hydrogen Peroxide Reduction Assay (EHRA).
To validate the EHRA for assessing both known antioxidant compounds and natural extracts.
To explore the applicability of EHRA across different solvent polarities.

Main Methods:

Utilized the Europium Tetracycline Hydrogen Peroxide Reduction Assay (EHRA) for antioxidant activity analysis.
Assessed known antioxidant compounds to establish stoichiometric relationships.
Tested various hop extracts (water, leaves, cones) to validate the method for natural products.

Main Results:

The EHRA accurately measures antioxidant activity via hydrogen peroxide scavenging.
A stoichiometric relationship was identified between antioxidants and scavenged oxidants.
Hop leaves demonstrated higher antioxidant activity than hop cones, with water extraction being most effective.
The EHRA proved effective in various solvents, bridging the hydrophilic/lipophilic gap.

Conclusions:

The EHRA is a robust, inexpensive, and accurate method for quantifying antioxidant activity.
The assay is suitable for diverse samples, including natural extracts like hops.
Hop leaves are a superior source of antioxidants compared to hop cones, particularly via water extraction.