Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The fluorescence-activating and absorption-shifting tag (FAST), a versatile protein marker for live plant cell imaging.

The Plant journal : for cell and molecular biology·2026
Same author

Advancing chloramphenicol detection through a novel porous organic polymer-enhanced screen-printed electrode.

Talanta·2026
Same author

Chemogenetic Modulation of Luciferase Emission Color for Imaging and Sensing.

ACS sensors·2026
Same author

Fluorescence Amplification Tags for Single-Molecule Imaging and Tracking of Proteins, RNA, and DNA in Live Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Synthesis of BODIPY@MOFs as Hybrid Materials for Emerging Applications: A Review.

Molecules (Basel, Switzerland)·2025
Same author

Clusterization-Induced Emission Shift in Copper(I) Thiones.

The journal of physical chemistry letters·2025

Related Experiment Video

Updated: May 8, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Luminogenic "clickable" lanthanide complexes for protein labeling.

Nicolas Candelon1, Niculina D Hădade, Mihaela Matache

  • 1Clermont-Université, Université Blaise Pascal and ENSCCF, Institut de Chimie de Clermont-Ferrand, F-63177 Aubière, France. arnaud.gautier@univ-bpclermont.fr.

Chemical Communications (Cambridge, England)
|September 3, 2013
PubMed
Summary

Researchers developed lanthanide-based luminescent "switch-on" systems using click chemistry. These systems enable versatile protein labeling for both general and specific activity-based applications.

More Related Videos

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

Related Experiment Videos

Last Updated: May 8, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

Area of Science:

  • Bioorganic chemistry
  • Chemical biology
  • Materials science

Background:

  • Lanthanide complexes offer unique luminescent properties.
  • Click chemistry, specifically azide-alkyne [3+2] cycloaddition, provides efficient conjugation methods.
  • Protein labeling is crucial for biological research and diagnostics.

Purpose of the Study:

  • To develop novel lanthanide-based luminescent "switch-on" probes.
  • To utilize these probes for effective protein labeling strategies.
  • To demonstrate their utility in both non-specific and activity-based protein labeling.

Main Methods:

  • Synthesis of lanthanide complexes functionalized for click chemistry.
  • Application of azide-alkyne [3+2] cycloaddition for probe conjugation.
  • Validation of luminescent "switch-on" behavior upon protein interaction.
  • Testing in non-specific and activity-based protein labeling assays.

Main Results:

  • Successful development of lanthanide-based luminescent "switch-on" systems.
  • Demonstration of efficient and selective protein labeling.
  • Effective application in activity-based protein profiling.
  • High signal-to-noise ratio achieved due to the "switch-on" mechanism.

Conclusions:

  • Lanthanide-based "switch-on" systems are effective tools for protein labeling.
  • The click chemistry approach allows for versatile probe design and application.
  • These systems offer potential for advanced biological imaging and diagnostics.