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Quantitative estimation of the total sulfide concentration in live tissues by two-photon microscopy
Chang Su Lim1, Sajal Kumar Das, Sun Young Yang
1Department of Chemistry, Korea University , 1-Anamdong, Seoul 136-701, Korea.
Analytical Chemistry
|September 4, 2013
Summary
Researchers developed TFCA, a novel two-photon probe, to visualize and quantify total sulfide levels in live tissues. This probe offers high selectivity and a significant signal increase, aiding in understanding sulfide
Area of Science:
- Biomedical Engineering
- Chemical Biology
- Neuroscience
Background:
- Hydrogen sulfide (H2S) is an emerging signaling molecule with protective roles against oxidative stress.
- Accurate detection and quantification of H2S and related species in biological systems remain challenging.
Purpose of the Study:
- To develop a ratiometric two-photon probe (TFCA) for visualizing total sulfide ([H2S] + [HS(-)]) distribution in live tissues.
- To establish a kinetic method for quantitatively measuring total sulfide concentration in live cells and tissues.
Main Methods:
- Synthesis and characterization of the ratiometric two-photon probe TFCA.
- Utilizing two-photon microscopy (TPM) for imaging sulfide distribution in live samples.
- Developing a kinetic approach based on TFCA's deazidation reaction to quantify sulfide concentration.
Main Results:
- TFCA exhibits a 110-fold intensity ratio increase upon reaction with sulfide species.
- The probe demonstrates high selectivity for hydrogen sulfide (HS(-)).
- Total sulfide concentrations were measured as near-zero in HeLa cells and 4-7 μM in rat colon tissues.
Conclusions:
- TFCA is a valuable tool for visualizing and quantifying total sulfide in live biological systems.
- The developed kinetic method provides a reliable way to measure sulfide levels in real-time.
- This work contributes to understanding the physiological roles of sulfide in different tissues.
