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Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
Published on: December 27, 2017
[Establishment of method detecting CD36 expression on human platelet and its application]
Ying Liu1, Xian-Guo Xu, Xiao-Fei Lan
1Institute of Transfusion Medicine, Blood Center of Zhejiang Province; Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|September 4, 2013
Summary
CD36 antigen deficiency on platelets, a cause of transfusion reactions, was found in 3.6% of Hangzhou apheresis donors. This finding aids research into platelet transfusion refractoriness.
Area of Science:
- Immunology
- Hematology
- Transfusion Medicine
Background:
- CD36 antigen deficiency on platelets increases the risk of anti-CD36 immune reactions.
- Platelet transfusion refractoriness (PTR) is a significant clinical challenge, partly due to immune reactions.
- Understanding CD36 antigen expression is crucial for managing transfusion complications.
Purpose of the Study:
- To determine the expression level of CD36 antigen on platelets in apheresis donors from the Hangzhou area.
- To analyze the frequency of CD36 deficiency in this population.
- To investigate the correlation between CD36 expression and platelet transfusion refractoriness.
Main Methods:
- Apheresis platelet donors' blood samples were collected.
- Platelet-rich plasma was isolated, and platelets were washed and adjusted.
- Flow cytometry was used to detect CD36 antigen expression using FITC-labeled CD36 and PE-labeled CD41 monoclonal antibodies.
Main Results:
- CD36 antigen deficiency was identified in 7 out of 192 apheresis platelet donors, a frequency of 3.6%.
- All identified deficiencies were classified as type II.
- Significant differences in CD36 antigen expression were observed, with 59 individuals showing low expression and 126 showing high expression.
Conclusions:
- The CD36 antigen deficient phenotype exists in the studied population.
- These findings provide valuable data on CD36 antigen distribution.
- This research contributes to understanding and potentially solving platelet transfusion refractoriness.
