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Updated: May 8, 2026

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
Published on: May 17, 2024
Molecular evolution of porcine reproductive and respiratory syndrome virus isolates from central China
Chunlong Mu1, Xiaoyan Lu, Erzhen Duan
1College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
To investigate the genetic diversity of prevailing porcine reproductive and respiratory syndrome virus (PRRSV) in Henan Province of China, 61 ORF5 gene sequences, originating from Henan Province during 2003-2010, were subjected to amino acid variation and phylogenetic analysis. The analyzed PRRSV ORF5 sequences carried evidence of one unique recombination event. Phylogenetic analysis revealed that all Henan isolates belonged to type 2 genotype and were divided into two subgroups. The dominant isolates had shifted from subgroup 1 to subgroup 2 during 2003-2010. Amino acid variation analysis of the glycoprotein 5 revealed that Henan PRRSV strains tended to accumulate more substitutions within the N-terminus and hypervariable region. Selective pressure analysis revealed evidence that some ORF5 sites have likely evolved in response to immune pressure.
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