Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tryptamine from wake-active monoaminergic neurons regulates sleep homeostasis.

Nature neuroscience·2026
Same author

Evolutionary convergence and divergence of hippocampal cytoarchitecture between rodents and primates revealed by single-cell spatial transcriptomics.

National science review·2026
Same author

Cortex-Restricted Deletion of Foxp1 Impairs Visual Responses by Disrupting Geniculocortical Connections in a Mouse Model of Autism.

Investigative ophthalmology & visual science·2026
Same author

A high-performance organic-inorganic composite bone cement enabled by a Sr-doped alginate-phosphate ternary system.

Journal of biomaterials applications·2026
Same author

Research on key indicators for diagnosis and prediction of rheumatoid arthritis based on GBDT+LR embedded feature selection model.

Frontiers in immunology·2026
Same author

FOXP2-Dependent Vulnerability of RGCs Under Acute Ocular Hypertension.

Molecular neurobiology·2025

Related Experiment Video

Updated: May 8, 2026

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
09:07

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Published on: March 17, 2014

Atlastin-1 regulates dendritic morphogenesis in mouse cerebral cortex.

Ying Gao1, Tian Jiang, Chunsheng Qu

  • 1Key Laboratory of Visual Science, National Ministry of Health and School of Optometry and Ophthalmology, Wenzhou Medical University, Wenzhou 325027, PR China.

Neuroscience Research
|September 4, 2013
PubMed
Summary

Atlastin-1 (ATL1) regulates neuron development in the brain. Its GTPase activity is crucial for dendritic growth, offering insights into hereditary spastic paraplegia SPG3A.

Keywords:
Atlastin-1Cerebral cortexIn utero electroporationMigrationNeuronal morphogenesis

More Related Videos

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
10:02

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Published on: December 14, 2015

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
09:25

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Related Experiment Videos

Last Updated: May 8, 2026

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
09:07

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Published on: March 17, 2014

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
10:02

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Published on: December 14, 2015

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
09:25

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Area of Science:

  • Neuroscience
  • Genetics
  • Developmental Biology

Background:

  • Hereditary spastic paraplegias (HSPs) are genetic disorders affecting lower limb function.
  • Mutations in atlastin-1 (ATL1) are linked to HSP SPG3A, but ATL1's brain function is unknown.
  • ATL1 mRNA is expressed in deep cortical layers during early mouse brain development.

Purpose of the Study:

  • To investigate the function of atlastin-1 (ATL1) in mammalian brain development.
  • To determine ATL1's role in cortical neuron morphology and migration.
  • To elucidate the neuropathogenic mechanisms of hereditary spastic paraplegia SPG3A.

Main Methods:

  • In utero electroporation to deliver ATL1 plasmids into mouse cortical neurons.
  • Assessing ATL1 function in cultured neurons and in situ during postnatal development.
  • Comparing the effects of wild-type ATL1 and a GTPase-deficient mutant (R217Q).

Main Results:

  • Overexpression of wild-type ATL1 enhanced dendrite growth and arborization in cortical neurons.
  • The GTPase-deficient ATL1 mutant (R217Q) did not promote dendritic growth.
  • Neither wild-type ATL1 nor the mutant affected cortical neuron migration.

Conclusions:

  • ATL1 regulates dendritic morphogenesis in a GTPase activity-dependent manner.
  • These findings suggest a role for ATL1 in neuronal development relevant to HSP SPG3A.
  • ATL1's function in dendritic development may be key to understanding SPG3A neuropathogenesis.