Related Experiment Video
Updated: May 8, 2026
A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage
Published on: April 28, 2022
Chlorhexidine as a factor that promotes peritoneal adhesions in rats with induced peritonitis
Paulo Roberto Rodrigues Bicalho1, Claudio Alvarenga Campos Mayrink, Fernando Fernandes
1Postgraduate Program in Surgery, Medical School, Federal University of Minas Gerais (UFMG), Belo Horizonte-MG, Brazil.
Purpose:
To investigate the effects of chlorhexidine on the formation of adhesions and dilation of the colon at the oral end of anastomosis in the presence of peritonitis.
Methods:
Peritonitis was induced in male Wistar rats by cecal ligation and puncture (CLP). Abdominal cavities were irrigated with tepid solutions containing 0.9% saline (SAL group; n=8) or 0.05% chlorhexidine (CHD group; n=8), after which colon anastomoses were performed. Control rats (n=8) were submitted to colon anastomoses but not to CLP. Animals were euthanised seven days after surgery and the incidence of adhesions, the degree of dilation of colon loops and an index were calculated to determine variables correlation.
Results:
No animals exhibited macroscopic signs of residual peritonitis or abdominal abscesses. Adhesions were observed in 75% of control and 100% of SAL and CHD animals. Dilation of intestinal loops at the oral end of anastomosis was observed in control (50%), SAL (57.2%) and CHD (66.7%) animals. The calculated index was 1.25 in group A; 1.86 in group B; and 2.0 group C.
Conclusion:
Chlorhexidine promoted stronger adhesions and a greater dilatation of colonic loops than control group.
