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Updated: May 8, 2026
Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model
Published on: April 2, 2017
Study of hepatocellular function in the murine model following hepatic artery selective clamping
José Guilherme Tralhão1, Ana Margarida Abrantes, Ana Cristina Gonçalves
1Faculty of Medicine, University of Coimbra, Surgery A, Surgery Department, CHUC, Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Coimbra, Portugal. jglrt@hotmail.com
Purpose:
To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function.
Methods:
Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability.
Results:
A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species.
Conclusions:
The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function.
