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Updated: May 8, 2026
Creation of Colonic Anastomosis in Mice
Published on: January 17, 2019
The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats
Leonardo de Castro Durães1, Igor Eduardo Caetano de Farias, Pedro Henrique Alves de Morais
1Postgraduate Program in Medical Sciences, School of Medicine,, University of Brasilia (UnB), Brasilia-DF, Brazil.
Purpose:
To investigate the effects of pneumoperitoneum on colonic anastomosis healing.
Methods:
Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Histopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed.
Results:
Breaking strength between groups: (day 3, p=0.165; day 7, p=0.219; day 14, p=0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p=0.006), greater infiltration of polymorphonuclear cells (p=0.001) and greater necrosis (p=0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p<0.001) on day 7 and an increase in groups I and II on day 14 (p<0.001). Hydroxyproline concentration was similar for groups on days 3 (p=0.152), 7 (p=0.913) or 14 (p=0.981).
Conclusion:
Carbon dioxide does not impair the healing of colonic anastomosis in rats.
