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Long non-coding RNA HNF1A-AS1 regulates proliferation and migration in oesophageal adenocarcinoma cells
Xue Yang1, Jee Hoon Song, Yulan Cheng
1Division of Gastroenterology, Department of Medicine, Johns Hopkins University School of Medicine, , Baltimore, Maryland, USA.
Gut
|September 4, 2013
Summary
Long non-coding RNA HNF1A-AS1 is upregulated in oesophageal adenocarcinoma (EAC). Its knockdown inhibits EAC cell growth and metastasis, potentially via chromatin assembly and H19 regulation.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are implicated in cancer development.
- The specific roles of lncRNAs in oesophageal adenocarcinoma (EAC) pathogenesis remain largely unknown.
Purpose of the Study:
- To identify functional lncRNAs in EAC.
- To elucidate the downstream mechanisms of dysregulated lncRNAs in EAC progression.
Main Methods:
- RNA-sequencing to identify upregulated lncRNAs in EAC.
- Quantitative RT-PCR for validation in patient tissues.
- Cellular assays and siRNA knockdown to assess lncRNA function.
Main Results:
- HNF1A-AS1 was significantly upregulated in EAC tissues.
- HNF1A-AS1 knockdown inhibited EAC cell proliferation, migration, and invasion.
- Knockdown affected chromatin/nucleosome assembly genes and reduced H19 expression.
Conclusions:
- HNF1A-AS1 is abnormally upregulated in human EAC.
- Dysregulation of HNF1A-AS1 contributes to oesophageal tumorigenesis.
- Mechanisms involve chromatin assembly modulation and H19 induction.
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