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[Formation of microbial biofilms in causative agents of acute and chronic pyelonephritis]

L V Lagun, Iu V Atanasova, D V Tapal'skiĭ

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |September 5, 2013
    PubMed
    Abstract

    Insights

    Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus form biofilms, contributing to chronic urinary tract infections. Biofilm formation is more intense in chronic cases and with co-existing urolithiasis.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Urology

    Background:

    • Microbial biofilms play a critical role in persistent and recurrent urinary tract infections (UTIs).
    • Understanding the biofilm-forming capacity of key uropathogens is crucial for managing pyelonephritis.

    Purpose of the Study:

    • To investigate the biofilm formation intensity of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus strains.
    • To correlate biofilm formation with different clinical forms of pyelonephritis.

    Main Methods:

    • Studied 150 clinical isolates from patients with acute and chronic pyelonephritis.
    • Quantified biofilm formation using crystal violet staining and spectrophotometric analysis.

    Main Results:

    • Pseudomonas aeruginosa exhibited the highest biofilm-forming ability, 2-3 times greater than Staphylococcus and Enterobacteriaceae.
    • Isolates from chronic pyelonephritis showed significantly higher biofilm formation than those from acute cases.
    • Microorganisms causing pyelonephritis associated with urolithiasis demonstrated enhanced biofilm formation.

    Conclusions:

    • Biofilm formation capacity is influenced by both the microbial species and the characteristics of the infectious process.
    • Intensive biofilm formation by these uropathogens may be a key factor in the chronicity of UTIs.

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