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Updated: May 8, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Receptor trafficking hypothesis revisited: plasticity of AMPA receptors during established status epilepticus
Karthik Rajasekaran1, Suchitra Joshi, Maxim Kozhemyakin
1Department of Neurology, University of Virginia, Health Sciences Center, Charlottesville, VA, U.S.A.
Abstract:
Status epilepticus (SE) is associated with a dynamic plasticity of postsynaptic neurotransmitter receptors. The plasticity of AMPA receptor (AMPAR)-mediated glutamatergic transmission during established SE (ESE), after development of benzodiazepine resistance, was evaluated. There was increased frequency and inward rectification of AMPAR-mediated excitatory postsynaptic currents at Schaffer collateral - CA1 pyramidal neuron synapses during ESE. Surface expression of the GluA1 subunit increased, and this was a consequence of N-methyl-d-aspartate receptor activation. Further, diminishing glutamate release by activation of somatostatin receptors prevented SE. These studies suggest that AMPAR-mediated glutamatergic transmission is strengthened during ESE.
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