Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Microbes and Methanogenesis01:26

Microbes and Methanogenesis

Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...
Segregation in Fresh Concrete01:16

Segregation in Fresh Concrete

Segregation in fresh concrete is a phenomenon where the components of the concrete mix separate, leading to uneven distribution and compromised structural integrity. This separation typically occurs when concrete is subjected to excessive horizontal movement within forms, or when it is dropped from considerable heights or forced through narrow, winding paths. As a result, heavier coarse aggregate particles settle at the bottom, while lighter, finer materials such as cement and water rise to the...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Making waves: Multicriteria strategies are key to reimagine the future of sewage sludge processing in Europe.

Water research·2026
Same author

Substrate biochemical composition does not impact hydrogen consumption rate but metabolite production during batch in-situ biomethanation process.

New biotechnology·2026
Same author

Corrigendum to "Modeling thermophilic syntrophic VFA oxidation using thermodynamic principles: Insights from enrichment cultures" [Bioresour. Technol. 449 (2026) 134365].

Bioresource technology·2026
Same author

Modeling thermophilic syntrophic VFA oxidation using thermodynamic principles: Insights from enrichment cultures.

Bioresource technology·2026
Same author

Coupling computational fluid dynamics and kinetic models using a compartmental model applied to a full-scale agricultural digester.

Journal of environmental management·2026
Same author

Microplastics in Brazilian rivers: An overview and a study of floating particle accumulation on the coast of Santa Catarina state.

The Science of the total environment·2025

Related Experiment Video

Updated: May 8, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
11:31

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

Published on: July 13, 2012

Liquid mixing and solid segregation in high-solid anaerobic digesters.

Hassen Benbelkacem1, Diana Garcia-Bernet2, Julien Bollon1

  • 1Université de Lyon, INSA-Lyon, Laboratoire de Génie Civil et d'Ingénierie Environnementale, 9, rue de la Physique, Villeurbanne cedex F-69621, France.

Bioresource Technology
|September 5, 2013
PubMed
Summary
This summary is machine-generated.

Increasing total solids (TS) content in dry anaerobic digesters improves macro-mixing and prevents particle segregation. Higher TS content shifts the digester

Keywords:
High-solid anaerobic digestionMixingResidence Time DistributionSedimentationTotal solid content

More Related Videos

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
07:34

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production

Published on: June 15, 2014

Related Experiment Videos

Last Updated: May 8, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
11:31

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

Published on: July 13, 2012

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
07:34

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production

Published on: June 15, 2014

Area of Science:

  • Biochemical Engineering
  • Waste Management
  • Environmental Science

Background:

  • Dry anaerobic digestion (DAD) is a key technology for biogas production from solid organic waste.
  • Understanding macro-mixing is crucial for optimizing DAD reactor performance and efficiency.
  • Previous studies have highlighted challenges in solid phase mixing and particle segregation within DAD systems.

Purpose of the Study:

  • To characterize the macro-mixing behavior of both liquid and solid phases in a laboratory-scale DAD.
  • To investigate the influence of waste origin and total solids (TS) content on mixing and particle segregation.
  • To identify key parameters governing particle segregation mechanisms in DAD.

Main Methods:

  • Utilized the Residence Time Distribution (RTD) technique with appropriate tracers to analyze macro-mixing.
  • Experimentally varied waste origin and total solids (TS) content (22% to 30% w/w).
  • Investigated the segregation of particles with different densities in the solid phase RTD.

Main Results:

  • Increased TS content from 22% to 30% (w/w) promoted a macro-mixing mode closer to a Plug Flow Reactor (PFR).
  • Particle segregation, particularly of dense particles, was observed at lower TS content.
  • Yield stress was identified as a critical parameter influencing particle segregation, with high yield stress stabilizing dense particles and preventing settling.

Conclusions:

  • Operating DAD at higher TS content enhances macro-mixing and approximates plug flow behavior.
  • Elevated TS content and corresponding high yield stress effectively prevent the sedimentation of denser particles.
  • Optimizing TS content is a viable strategy to improve solid phase mixing and mitigate segregation issues in DAD.