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Updated: May 8, 2026
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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
A cost-effective method for high-throughput construction of illumina sequencing libraries
Joseph P Dunham1, Maren L Friesen
1Section of Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA. jpdunham@usc.edu
Cold Spring Harbor Protocols
|September 5, 2013
Summary
This study presents a cost-effective, high-throughput DNA library preparation method. The optimized protocol significantly reduces costs for next-generation sequencing, making large-scale studies more accessible.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- Next-generation sequencing (NGS) costs are decreasing, but library preparation remains expensive.
- High costs limit large-scale comparative and experimental studies.
- Multiplexing many samples necessitates efficient and affordable library preparation.
Purpose of the Study:
- To develop a cost-effective and time-efficient high-throughput method for DNA library preparation.
- To reduce the financial burden of NGS library construction for large sample numbers.
Main Methods:
- Optimized and scaled-down library construction steps.
- Utilized commonly available reagents and a 96-well format.
- No specialized equipment required.
Main Results:
- Achieved a 10-fold cost reduction compared to commercial kits.
- Per-library costs reduced to ~$12.60-14.90 (individual) or ~$8.60-10.60 (pooled).
- Enabled pooling of up to 144 samples per lane using tested barcodes.
Conclusions:
- The optimized protocol offers substantial savings in reagent cost and personnel hours.
- This high-throughput method makes large-scale genomic studies more feasible.
- The protocol provides a scalable and economical solution for DNA library preparation.
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