Simultaneous visualization of multiple protein interactions using multicolor bimolecular fluorescence complementation (BiFC) analysis

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Updated: May 8, 2026

Bimolecular Fluorescence Complementation
08:54

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

Tom K Kerppola

    Cold Spring Harbor Protocols
    |September 5, 2013
    PubMed

    Abstract:

    This multicolor bimolecular fluorescence complementation procedure is designed for the analysis of alternative protein interactions in cultured mammalian cells, but it can be readily adapted to any cell type or aerobically grown organism that can be genetically modified to express the fusion proteins.

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