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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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Related Experiment Video

Updated: May 8, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

Human origin recognition complex binds preferentially to G-quadruplex-preferable RNA and single-stranded DNA.

Shoko Hoshina1, Kei Yura2, Honami Teranishi1

  • 1From the Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo 112-8681, Japan.

The Journal of Biological Chemistry
|September 5, 2013
PubMed
Summary

Human origin recognition complex (ORC) binds preferentially to G-rich RNA and single-stranded DNA, particularly G-quadruplex structures. This sequence preference in human ORC may explain its role in DNA replication origin recognition.

Keywords:
DNA MethyltransferaseDNA ReplicationDNA-binding ProteinG-quadruplexORCProtein StructureRNA-binding ProteinReplication Origin

Related Experiment Videos

Last Updated: May 8, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The origin recognition complex (ORC) is crucial for initiating DNA replication in eukaryotes.
  • Unlike yeast ORC, metazoan ORC lacks strict sequence specificity for DNA binding.

Purpose of the Study:

  • To investigate the sequence specificity of human ORC binding.
  • To identify the molecular basis for human ORC's DNA/RNA binding preferences.

Main Methods:

  • Characterization of human ORC binding to G-rich RNA and single-stranded DNA (ssDNA).
  • Mapping of the G-rich RNA-binding domain within the ORC1 subunit.
  • Structure modeling of the ORC1 binding domain.

Main Results:

  • Human ORC preferentially binds to G-quadruplex (G4)-preferable G-rich RNA and ssDNA.
  • A specific domain in the ORC1 subunit mediates this G4-preferable binding.
  • This binding preference aligns with sequences found at human replication origins.

Conclusions:

  • Human ORC exhibits sequence specificity in binding RNA and ssDNA, unlike its double-stranded DNA binding.
  • The identified ORC1 domain provides a structural basis for this specificity.
  • Findings offer new perspectives on metazoan ORC function and regulation in DNA replication.