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A comparative study on anti-hyperalgesia effect of MTA and Ketoprofen in inflammatory pain
Fatemeh Abbasipour1, Hengameh Bakhtiar, Mehdi Vatanpour
1Dental Student, Neuroscience Research Centre and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences and Endodontic Department, Dental School, Islamic Azad University of Medical Sciences, Tehran, Iran.
Introduction:
Mineral trioxide aggregate (MTA) is an endodontic material with different clinical applications e.g. root-end filling, pulp capping and perforation repair. It has been reported to possess antimicrobial and antifungal activities. The aim of this study was to examine the effect of White MTA on formalin-induced hyperalgesia in a rat with inflammatory pain.
Materials And Methods:
Inflammatory pain was induced by subcutaneous (SC) injection of formalin (40 µL, 2.5%) into the rat upper lip. The nociceptive behavioral responses i.e. shaking of the lower jaw and face rubbing were quantified. 40 µL of eugenol (50 mg/kg), WMTA (20 mg/0.2 mL) or ketoprofen were injected solely or in combination with formalin 2.5% and the behavioral responses were compared with those observed after formalin treatment alone. One-way ANOVA, Tukey were used for analysis of data.
Results:
Formalin 2.5% provoked a biphasic nociceptive response, with an early and short lasting first tonic phase followed by a second phase. Solely SC injection of either WMTA or ketoprofen (a non steroidal anti-inflammatory drug) did not stimulate any significant nociceptive behaviour. However, injection of eugenol (a pain relieving agent) induced the early phase not the tonic phase of nociceptive response. WMTA, eugenol or ketoprofen injection 20 min before formalin injection attenuated the first phase but somehow prevented the induction of the second phase of nociceptive responses which were produced by formalin. Behavioural nociceptive responses including shaking of the lower jaw and face rubbing were significantly reduced when the subject was pretreated with either WMTA or ketoprofen (P<0.001).
Conclusion:
In this study, WMTA induced pain reduction by suppression of the formalininduced nociceptive response.
Insights
White Mineral Trioxide Aggregate (WMTA) effectively reduced inflammatory pain in rats by suppressing formalin-induced nociceptive responses. This endodontic material demonstrated significant pain-reducing effects, similar to ketoprofen.
Area of Science:
- Endodontics
- Pain Management
- Pharmacology
Background:
- Mineral Trioxide Aggregate (MTA) is a versatile endodontic material.
- MTA exhibits known antimicrobial and antifungal properties.
- This study investigates White MTA's (WMTA) effect on inflammatory pain.
Purpose of the Study:
- To evaluate the analgesic effect of WMTA on formalin-induced hyperalgesia in a rat model.
- To compare WMTA's efficacy with known analgesics like ketoprofen and eugenol.
Main Methods:
- Inflammatory pain was induced via subcutaneous formalin injection in rats.
- Nociceptive behaviors (jaw shaking, face rubbing) were quantified.
- WMTA, ketoprofen, or eugenol were administered before formalin to assess pain modulation.
Main Results:
- Formalin induced a biphasic nociceptive response.
- WMTA and ketoprofen significantly attenuated both phases of formalin-induced pain.
- WMTA demonstrated significant pain reduction (P<0.001).
Conclusions:
- White MTA effectively suppresses formalin-induced nociceptive responses.
- WMTA exhibits significant analgesic properties in an inflammatory pain model.
- WMTA shows potential as an adjunct in pain management therapies.
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