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Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
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Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay

Published on: April 29, 2020

[Optimization of a method for accounting the results of enzyme immunoassay for toxocariasis]

A S Nechaeva, T V Starkova, E A Chernikova

    Meditsinskaia Parazitologiia I Parazitarnye Bolezni
    |September 6, 2013
    PubMed
    Abstract

    No abstract available in PubMed .

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    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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