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Refolding of chicken cystatin and human stefin A studied by fast protein liquid chromatography (FPLC)
E Zerovnik1, J Kos, B Lenarcic
1Dept. Biochemistry, J. Stefan Institute, Ljubljana.
Abstract:
Denaturant gradient gel-chromatography [Endo, S. et al. (1983) Anal. Biochem. 131, 108-120] has been used to follow renaturation of chicken cystatin and human stefin A. Slow gradient of GuHCl was applied to the size-exclusion Superose 12 column. The column was maintained at 5 degrees C by circulating water from a cryostate. When the denatured protein has been injected it started to refold on the column. By the simplified equation, according to [Hanai, R. et al. (1986) Biophys. Chem. 25, 27-36] approximate kinetics of folding was obtained. No intermediate species in folding could be detected from 6M to 2M GuHCl, only the close-to-denatured state and the close-to-native state.