Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dental amalgams and blood mercury concentrations in American adults.

Clinical chemistry and laboratory medicine·2025
Same author

Tooth decay prevention and neurodevelopmental disorder risk following childhood fluoride exposure.

BMC pediatrics·2025
Same author

Constitutional chromosomal anomalies in children, fetal alcohol syndrome, and maternal toxicant exposures: A longitudinal cohort study.

Mutation research. Genetic toxicology and environmental mutagenesis·2024
Same author

Estimated mercury vapor exposure from amalgams among American pregnant women.

Human & experimental toxicology·2024
Same author

Reductions in plasma and urine mercury concentrations following N,N'bis-(2-mercaptoethyl) isophthalamide (NBMI) therapy: a post hoc analysis of data from a randomized human clinical trial.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine·2023
Same author

Colon Cancer Risk Following Intestinal <i>Clostridioides difficile</i> Infection: A Longitudinal Cohort Study.

Journal of clinical medicine research·2023

Related Experiment Video

Updated: May 8, 2026

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models
08:48

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models

Published on: June 30, 2023

L-carnitine exposure and mitochondrial function in human neuronal cells.

David A Geier1, Mark R Geier

  • 1The Institute of Chronic Illnesses, Inc., 14 Redgate Ct., Silver Spring, MD, 20905, USA.

Neurochemical Research
|September 6, 2013
PubMed
Summary

L-Carnitine enhances mitochondrial function in human neuronal cells. This study found optimal L-carnitine concentrations improve cellular energy production, suggesting potential for cognitive health.

More Related Videos

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
09:56

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

Published on: May 23, 2011

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
04:50

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Published on: June 17, 2011

Related Experiment Videos

Last Updated: May 8, 2026

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models
08:48

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models

Published on: June 30, 2023

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
09:56

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

Published on: May 23, 2011

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
04:50

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Published on: June 17, 2011

Area of Science:

  • Cellular Biology
  • Neuroscience
  • Biochemistry

Background:

  • L-Carnitine is essential for mammalian energy metabolism, facilitating fatty acid transport into mitochondria for energy production.
  • Clinical trials suggest L-Carnitine may improve cognitive function and support healthy cognitive aging.
  • Mitochondrial dysfunction is implicated in various neurological conditions.

Purpose of the Study:

  • To investigate the effect of L-Carnitine on mitochondrial function in human neuronal and astrocytoma cell cultures.
  • To determine the effective concentration range of L-Carnitine for enhancing cellular mitochondrial activity.

Main Methods:

  • Human neuroblastoma (SH-SY-5Y) and astrocytoma (1321N1) cells were cultured and treated with varying concentrations of L-Carnitine hydrochloride.
  • Mitochondrial function was measured using a colorimetric cell assay kit.
  • Absorbance was read using a microplate reader to quantify mitochondrial activity.

Main Results:

  • Significant increases in mitochondrial function were observed in both cell types when exposed to L-Carnitine concentrations ranging from 100 nM to 100 μM.
  • No significant positive effects on mitochondrial function were noted at lower or higher L-Carnitine concentrations.
  • The study identified a specific effective dose range for L-Carnitine's impact on cellular energy metabolism.

Conclusions:

  • Acute administration of L-Carnitine can significantly enhance mitochondrial function in human neuronal and astrocytoma cells within a specific concentration window.
  • These findings support the therapeutic potential of L-Carnitine for improving neuronal function.
  • Further research is recommended to explore L-Carnitine derivatives and various markers of mitochondrial function in different in vitro models.