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A model to explain the osmotic pressure behavior of hemoglobin and serum albumin
1Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7800.
Abstract:
Previously published osmotic pressure data on hemoglobin and bovine serum albumin were used to determine the osmotically unresponsive solvent volume per unit dry mass of protein. A model is presented that accounts for the osmotic pressure of globular proteins based on a surface-associated osmotically unresponsive solvent volume. The model also accounts for changes in the osmotically unresponsive solvent volume owing to changes in pH, cosolute salt concentration, protein conformation, and protein aggregation.