Related Experiment Video
Updated: May 8, 2026

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
Published on: March 24, 2023
[Possibility of the plasmid transfer within bacteria - compounds of the sulfidogenic microbial community]
Abstract:
Extrachromosomal elements have been found within the isolates of ammonifying and iron-reducing bacteria obtained from the natural sulfidogenic community. These elements were small with size approximately 5-9 kb. Transconjugant strains Pseudomonas aeruginosa 27, P aeruginosa 28, P mendocina 29, Aeromonas hydrophila/caviae 30, harboured plasmids RP4 and R68.45 were obtained. The conjugation rate and retention of the plasmid within transconjugants were studied. P mendocina 29 cells with conjugation rate - 3.0*10(-6) CFU/ recipient cell, and P. aeruginosa 27 with rate 2.4*l0(-7) CFU/recipient cell were the most susceptible to R68.45 plasmid. The most stable retention is shown for transconjugated strains such as P aeruginosa 27 (R68.45), P. aeruginosa 27(RP4), P. mendocina 29(R68.45), A. hydrophila/caviae 30(RP4), A. hydrophila/caviae 30(R68.45). Thus, it was experimentally shown that corrosive-relevant bacteria form sulfidogenic microbial community which is able to accept extrachromosomal genetic elements from other bacteria when they act as recipients in conjugational process.
Related Concept Videos
Plasmids
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Conjugation
Mechanism of Conjugation
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...

