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Published on: April 9, 2016
Characterization and flocculation properties of a carbohydrate bioflocculant from a newly isolated Bacillus
Sahar A Zaki1, Marwa F Elkady, Soha Farag
1Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, 21934, Egypt.
Abstract:
In this study, a bioflocculant with a high flocculation activity (> 98%) produced by strain 40B, which was isolated from a brackish water was investigated By 16S rDNA sequence analysis, strain 40B was identified as Bacillus velezensis. Chemical analysis of the bioflocculant 40B indicated that it contained 2% protein and 98% carbohydrates. FTIR analysis showed the presence of carboxyl, hydroxyl and amino groups, which were preferred for the flocculation process. The optimal concentration for the flocculation activity was 3.5 mg (-1). This polysaccharide could also flocculate kaolin suspension over a wide range of pH (1-10) and temperature (5-85 degrees C) in the presence of CaCl2. The stability of the bioflocculant 40B under various conditions suggests its possible use in the industries and environmental applications. However, no previous report exists on the isolation and characterization of a bioflocculant from the Bacillus velezensis.
