Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DAMP Laden Extracellular Vesicles From the Airways of Patients With Severe SARS-CoV-2 Respiratory Infection Compromise Inflammation and Cellular Metabolism.

Journal of extracellular vesicles·2026
Same author

Heparan Sulphate Glycosaminoglycan Chains Contribute to the Tethering of Coronal Factors and Are Important for Extracellular Vesicle-Mediated Fibroblast Activation.

Journal of extracellular biology·2026
Same author

Unravelling nanoscale chemistries in complex biological systems using photoinduced force microscopy (PiFM).

Faraday discussions·2026
Same author

Extracellular vesicle-mediated delivery of miR-181a-3p confers neuroprotection to degenerating retinal ganglion cells.

Experimental eye research·2026
Same author

Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI.

eLife·2025
Same author

Antibiotic carry over is a confounding factor for cell-based antimicrobial research applications.

Scientific reports·2025
Same journal

Silencing the Signal: The Metastasis Suppressor NDRG1 Disrupts Small Extracellular Vesicle-Mediated Crosstalk in Pancreatic Cancer.

Journal of extracellular vesicles·2026
Same journal

Integrating Nuclear Magnetic Resonance into Bioprocess Control of Clinical-Grade Extracellular Vesicle Manufacturing.

Journal of extracellular vesicles·2026
Same journal

Eliminating PD-L1 on Dendritic Cell Extracellular Vesicles for Immunotherapy Potentiates Immune-Mediated Tumour Rejection in Mice.

Journal of extracellular vesicles·2026
Same journal

Regulatory Challenges and Opportunities for Cell-Derived Extracellular Vesicles in Pharmaceutical Development: A European and Global Perspective.

Journal of extracellular vesicles·2026
Same journal

ISEV2026 Abstract Book.

Journal of extracellular vesicles·2026
Same journal

Label-Free Clustering Analysis Platform Drives Cascaded Workflow for Scalable Production of Therapeutic Extracellular Vesicles.

Journal of extracellular vesicles·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
11:30

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Published on: September 16, 2022

How pure are your vesicles?

Jason Webber1, Aled Clayton

  • 1Institute of Cancer and Genetics, School of Medicine, Velindre Cancer Centre, Cardiff University, Cardiff, UK.

Journal of Extracellular Vesicles
|September 7, 2013
PubMed
Summary
This summary is machine-generated.

We present a simple method to assess vesicle purity by comparing nano-vesicle counts to protein concentration. This quality control approach aids researchers in standardizing vesicle preparations and evaluating biomarkers.

Keywords:
extracellular vesiclesnano-particle trackingsample purityvesicle counting

More Related Videos

Isolation and Characterization of Cyanobacterial Extracellular Vesicles
08:44

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
07:15

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
11:30

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Published on: September 16, 2022

Isolation and Characterization of Cyanobacterial Extracellular Vesicles
08:44

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
07:15

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Biotechnology

Background:

  • Vesicle preparations are crucial in various biological and biotechnological applications.
  • Assessing the purity of vesicle preparations is essential for reliable experimental outcomes.
  • Current methods for vesicle purity assessment can be complex or time-consuming.

Purpose of the Study:

  • To propose a straightforward and routine method for estimating vesicle preparation purity.
  • To establish criteria for defining pure vesicles.
  • To facilitate the comparison and standardization of vesicle purity across different studies.

Main Methods:

  • Utilizing the NanoSight platform for nano-vesicle counting.
  • Employing a colorimetric protein assay, such as the BCA assay, for protein concentration measurement.
  • Calculating the ratio of nano-vesicle counts to protein concentration.

Main Results:

  • A simple ratio of nano-vesicle counts to protein concentration can effectively estimate vesicle purity.
  • The proposed method is suitable for routine quality control in research laboratories.
  • The approach provides a basis for standardizing purity assessments across diverse studies.

Conclusions:

  • The proposed method offers a practical and accessible approach to assess vesicle purity.
  • This quality control measure is particularly important for evaluating vesicular biomarkers.
  • Implementing this method can enhance the reproducibility and comparability of vesicle-based research.