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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
10:10

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Published on: November 2, 2018

Ultrahigh speed spectral-domain optical coherence microscopy.

Hsiang-Chieh Lee1, Jonathan J Liu, Yuri Sheikine

  • 1Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Biomedical Optics Express
|September 7, 2013
PubMed
Summary

We developed a fast spectral-domain optical coherence microscopy (SD-OCM) system for multiscale imaging. This system achieves high speeds and resolutions for detailed cellular imaging of biological specimens.

Keywords:
(170.3880) Medical and biological imaging(170.4500) Optical coherence tomography(170.6900) Three-dimensional microscopy(180.1790) Confocal microscopy

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Area of Science:

  • Biomedical Optics
  • Microscopy
  • Medical Imaging

Background:

  • Optical coherence microscopy (OCM) enables high-resolution imaging of biological tissues.
  • Advancements in speed and resolution are crucial for multiscale and in vivo applications.

Purpose of the Study:

  • To demonstrate a compact, ultrahigh-speed spectral-domain OCM (SD-OCM) system for multiscale imaging.
  • To evaluate the system's performance with interchangeable objectives and assess imaging capabilities on biological specimens.

Main Methods:

  • Utilized a high-speed 512-pixel line scan camera for rapid data acquisition.
  • Employed interchangeable water immersion objectives (10×, 20×, 40×) for varying magnifications.
  • Implemented image mosaicking for extended field-of-view imaging.

Main Results:

  • Achieved an imaging speed of 210,000 A-scans per second.
  • Demonstrated cellular-resolution en face imaging with axial resolution of ~4.2 µm and transverse resolutions down to ~1.1 µm.
  • Successfully imaged normal and pathological human colon and kidney specimens ex vivo.

Conclusions:

  • The developed SD-OCM system offers a versatile platform for multiscale, high-speed, and high-resolution imaging.
  • The system's performance characteristics are suitable for detailed analysis of biological structures.
  • This technology aids in the design and evaluation of Fourier domain OCM systems across different resolution regimes.