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Updated: May 8, 2026
Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
Published on: April 14, 2023
[Study on the effect of extracts from the leaves of Phyllanthus emblica on immune function of mice]
Zhen-Guo Zhong1, Xue-Fei Luo, Jin-Lan Huang
1Center of Research & Development of New Drugs, Guangxi University of Chinese Medicine, Nanning 530001, China. zhongzg@gxtcmu.edu.cn
Objective:
To explore the effect of extracts from the leaves of Phyllanthus emblica (PLFs) on the immune function of mice.
Methods:
70 Kunming mice were choosed to conduct the acute toxicity test of PLFs. The mice were randomly divided into four groups: PLFs high-dosage group, mid-dosage group, low-dosage group and control group. The high,mid,low-dosage groups were treated with PLFs 1.982, 0.991 and 0.496 g/kg respectively per day. The same volume of double distilled water was given to the control group. All by intragastric administration for 7 d. The animals were killed and indexes of thymus and spleen were calculated. The expurgation index K and phagocyte index a were detected after the mice being injected with a dilute India ink through caudal vein. In addition, prepared spleen cells conventionally,the activity of Natural Killer cells was measured and the proliferation of T and B cells were detected. The effect of the extracts on serum hemolysin was detected after the SRBC was injected into the enterocoelia.
Results:
The LD50 of PLFs was 9. 911 g/kg. Compared with the control group, the indexes of thymus and spleen in the treatment groups had no markedly difference (P > 0.05). The high- and mid-dosage groups could obviously improve the expurgation index K (P < 0.05), phagocyte index alpha (P < 0.05) and NK cell activity (P < 0.05).
Conclusion:
The extracts from Phyllanthus emblica leaves can promote nonspecific immunity immune function in mice.
