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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

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Related Experiment Video

Updated: May 8, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Cell-surface Notch1 expression identifies a primitive phenotype within CD34+ CD38- haematopoietic cells.

Virginia Portillo1, Nicholas Chadwick, Ruth Lloyd

  • 1Faculty of Life Sciences, University of Manchester, Manchester, UK.

European Journal of Haematology
|September 10, 2013
PubMed
Summary
This summary is machine-generated.

Notch1 expression on cord blood CD34(+) cells identifies a unique stem cell population. This Notch1-positive subset shows enhanced self-renewal and multilineage potential, suggesting its utility as a novel stem cell marker.

Keywords:
CD34+Notchcord bloodhaematopoieticprogenitor

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Last Updated: May 8, 2026

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Published on: February 28, 2017

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Notch signalling is crucial for hematopoietic stem cell (HSC) self-renewal.
  • Previous studies explored Notch pathway activation/inhibition but lacked functional characterization of Notch1 expression.
  • Understanding Notch1's role in HSC function is vital for regenerative medicine.

Purpose of the Study:

  • To characterize human cell-surface Notch1 expression in cord blood (CB) CD34(+) cells.
  • To investigate the functional implications of Notch1 expression in CD34(+) cells in vitro.
  • To determine if Notch1 can serve as a marker for primitive hematopoietic stem cells.

Main Methods:

  • Developed a monoclonal antibody for Notch1 extracellular domain detection.
  • Assessed Notch1 expression on CB CD34(+) cells using flow cytometry.
  • Sorted CD34(+) CD38(-) cells based on Notch1 expression and cultured them to evaluate stem cell activity and multilineage potential via CAFC assays.

Main Results:

  • Identified cell-surface Notch1 expression in primitive CD34(+) cell subsets, including CD34(+) CD38(-) cells.
  • The CD34(+) CD38(-) Notch1(+) population exhibited significantly enriched stem cell activity.
  • Notch1(+) cells demonstrated multilineage potential, unlike Notch1(-) counterparts.

Conclusions:

  • Notch1 is expressed on a functionally distinct CD34(+) cell subpopulation.
  • This Notch1-expressing subset is highly enriched for stem cell activity and multilineage potential.
  • Notch1 may serve as a novel marker for identifying potent hematopoietic stem cells.