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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
Published on: May 22, 2012
Explanatory chapter: PCR primer design
1Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Methods in Enzymology
|September 10, 2013
Abstract:
This chapter is intended as a guide on polymerase chain reaction (PCR) primer design (for information on PCR, see General PCR and Explanatory Chapter: Troubleshooting PCR). In the next section, general guidelines will be provided, followed by a discussion on primer design for specific applications. A list of recommended software tools is shown at the end.
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