Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Implementation Strategy to Develop Sustainable Surveillance Activities Through Adoption of a Target Operating Model.

Frontiers in public health·2022
Same author

Design of a synthetic yeast genome.

Science (New York, N.Y.)·2017
Same author

The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans.

Developmental biology·2016
Same author

SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes.

Genome research·2015
Same author

Total synthesis of a functional designer eukaryotic chromosome.

Science (New York, N.Y.)·2014
Same author

Saccharomyces cerevisiae growth media.

Methods in enzymology·2013

Related Experiment Video

Updated: May 8, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

PCR-based random mutagenesis.

Jessica S Dymond1

  • 1The High Throughput Biology Center and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Enzymology
|September 10, 2013
PubMed
Summary
This summary is machine-generated.

Random PCR mutagenesis quickly creates diverse mutant genetic elements affordably. This method accelerates the generation of genetic libraries for research and development.

Keywords:
BSAError-prone PCRMutagenesisMutagenic PCRPCR-based random mutagenesisTemplate DNA

More Related Videos

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Generating diverse mutant genetic elements is crucial for understanding gene function and developing new biotechnologies.
  • Traditional methods for creating mutant libraries can be time-consuming and expensive.

Purpose of the Study:

  • To present a rapid and inexpensive method for constructing libraries of mutant genetic elements.
  • To demonstrate the utility of random PCR mutagenesis for generating genetic diversity.

Main Methods:

  • Random PCR mutagenesis was employed to introduce mutations into target genetic elements.
  • The resulting mutant library was characterized to assess the diversity of generated mutations.

Main Results:

  • Random PCR mutagenesis successfully generated a library of mutant genetic elements.
  • The method proved to be rapid and cost-effective compared to existing approaches.

Conclusions:

  • Random PCR mutagenesis is an efficient technique for creating diverse mutant genetic element libraries.
  • This approach offers a valuable tool for accelerating genetic research and synthetic biology applications.