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Updated: May 8, 2026

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
Published on: October 21, 2017
[Quercetin regulates cell cycle-related gene expression in a model of glucose-oxygen deprivation in astrocytes]
Fang Yao1, Lanlan Zhang, Zhaohu Yuan
1Research Center of Clinical Medicine, Southern Medical University, Guangzhou, China. fangfang5087@126.com
Objective:
To study the effect of quercetin on gene expression in astrocytes after glucose-oxygen deprivation and the underlying mechanism.
Methods:
The primary cultured astrocytes were randomly divided into glucose-oxygen deprivation group (only treated with glucose-oxygen deprivation for 4 hours) and glucose-oxygen deprivation combined with quercetin-treated group (glucose-oxygen deprivation for 4 hours combined with quercetin treatment for 24 hours). Their mRNA expressions were analyzed by the large-scale oligo microarray. The differential genes obtained were further confirmed by real-time quantitative PCR (qRT-PCR).
Results:
Compared with the glucose-oxygen deprivation group, the glucose-oxygen deprivation combined with quercetin-treated group presented the changes in the expressions of 31 genes that were related to cell cycle, of which 5 genes were up-regulated and 26 were down-regulated. Six of those differential genes were confirmed by qRT-PCR and the result of their differential expressions was consistent with that by large-scale oligo microarray.
Conclusion:
Quercetin can regulate some of cell cycle-related genes in astrocytes after glucose-oxygen deprivation.
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