Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioplastics01:27

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
Microbial Bioremediation of Plastics01:28

Microbial Bioremediation of Plastics

Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oil-in-water Emulsion as a Strategy for Taste Masking of Cannabinoid Extract.

AAPS PharmSciTech·2026
Same author

Exploring rifamycin cytotoxic potential through targeted liposomal formulations.

International journal of pharmaceutics·2026
Same author

Expression, Solubilization, and Refolding Recovery of a Novel l‑Asparaginase-Arginase 1 Chimera from <i>E. coli</i> Inclusion Bodies.

ACS omega·2026
Same author

Tailored high-cell-density fed-batch cultivation and induction strategies for optimized production of a low-immunogenic L-asparaginase mutant in Escherichia coli.

Enzyme and microbial technology·2026
Same author

Cannabis: from crop to shop-some insights about stability to access quality control.

Journal of cannabis research·2026
Same author

Industrial <i>Cannabis</i>, Cannabic Residue or Industrial <i>Cannabis</i> Waste? Perspectives on the Utilization, Reutilization, and Recycling of <i>Cannabis</i>.

Cannabis and cannabinoid research·2026

Related Experiment Video

Updated: May 8, 2026

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

Polymer-based alternative method to extract bromelain from pineapple peel waste.

Letícia Celia de Lencastre Novaes1, Valéria de Carvalho Santos Ebinuma, Priscila Gava Mazzola

  • 1Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.

Biotechnology and Applied Biochemistry
|September 10, 2013
PubMed
Summary

This study optimized bromelain extraction from pineapple waste using an aqueous two-phase system. The process achieved a high yield and purification factor, demonstrating an efficient method for utilizing pineapple byproducts.

Keywords:
aqueous two-phase systembromelainpineapple wastepoly(acrylic acid)polyethylene glycol

More Related Videos

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
07:05

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Published on: August 23, 2024

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
12:00

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

Related Experiment Videos

Last Updated: May 8, 2026

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
07:05

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Published on: August 23, 2024

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
12:00

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

Area of Science:

  • Biochemistry
  • Biotechnology
  • Food Science

Background:

  • Bromelain, a proteolytic enzyme from pineapple (Ananas comosus Merr.), has diverse applications in medicine, food, and supplements.
  • Pineapple waste presents an underutilized resource with significant potential for bromelain recovery.
  • Enzyme extraction from agricultural byproducts offers environmental and economic benefits.

Purpose of the Study:

  • To optimize the extraction of bromelain from pineapple waste.
  • To investigate the use of an aqueous two-phase system (ATPS) for bromelain purification.
  • To evaluate the efficiency of polyethylene glycol (PEG) and poly(acrylic acid) in ATPS for bromelain recovery.

Main Methods:

  • Pineapple waste was used as the source for bromelain extraction.
  • An aqueous two-phase system composed of polyethylene glycol (PEG) and poly(acrylic acid) was employed.
  • Bromelain partitioning and purification factor were analyzed under optimized conditions.

Main Results:

  • Bromelain demonstrated a preferential partition to the polyethylene glycol (PEG)-rich phase in the ATPS.
  • The optimized extraction process achieved a maximum yield of 335.27%.
  • A purification factor of 25.78 was obtained, indicating significant purification of bromelain.
  • Statistical analysis confirmed the significance of all analyzed variables in the extraction process.

Conclusions:

  • The aqueous two-phase system (PEG/poly(acrylic acid)) is effective for bromelain extraction from pineapple waste.
  • This method offers a high yield and purification, making pineapple waste a viable source for commercial bromelain production.
  • Optimized enzyme extraction from agricultural byproducts is a sustainable approach with commercial potential.