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Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
Published on: January 21, 2019
Fluorescence microscopic visualization of non cellular components during initial bioadhesion in situ.
A Kensche1, S Basche, W H Bowen
1Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, D-01307 Dresden, Germany. Anna.Kensche@uniklinikum-dresden.de
Archives of Oral Biology
|September 10, 2013
Summary
New fluorescent labeling methods visualize early oral biofilm formation. This technique reveals interactions between pellicle enzymes, glucans, and bacteria, aiding in understanding caries susceptibility.
Area of Science:
- Oral microbiology
- Biomaterials science
- Microscopy techniques
Background:
- Oral biofilm formation begins with molecular interactions and bioadhesion.
- Understanding initial biofilm development is crucial for preventing dental caries.
Purpose of the Study:
- To develop fluorescent labeling protocols for simultaneous visualization of pellicle enzymes, extracellular glucans, and bacteria.
- To study the initial phase of intraoral biofilm formation in situ.
Main Methods:
- Biofilm samples were collected in situ from enamel and dentine slabs worn by subjects.
- Epifluorescent microscopy was used with specific antibody-based fluorescent labeling for pellicle enzymes.
- Combined DAPI/Concanavalin A staining identified adherent bacteria and glucans.
Main Results:
- Established fluorescent labeling protocols successfully visualized pellicle enzymes, glucans, and bacteria.
- Observed characteristic agglomeration of glucans and bacteria during early bioadhesion.
- Detected increased concentration of pellicle enzymes in the initial biofilm phase.
Conclusions:
- Fluorescent labeling techniques offer valuable insights into in situ biofilm determinant interactions.
- This methodology can help assess the impact of oral therapeutics on caries susceptibility.
