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Updated: May 8, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

TOPK and PTEN participate in CHFR mediated mitotic checkpoint

Swapnil R Shinde1, Narmadha Reddy Gangula, Sridhar Kavela

  • 1Laboratory of Cell Death & Cell Survival, Centre for DNA Fingerprinting and Diagnostics (CDFD), Nampally, Hyderabad 500001, India.

Cellular Signalling
|September 10, 2013
PubMed

Insights

The study reveals how CHFR (Check point protein with FHA and RING domains) regulates mitosis by controlling TOPK (a kinase) and PTEN (a phosphatase) levels, ensuring genomic stability.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genomic Stability

Background:

  • Mitotic progression requires coordinated protein action for genomic stability.
  • CHFR (Check point protein with FHA and RING domains) is an E3 ubiquitin ligase regulating mitotic entry.
  • The precise molecular mechanisms of CHFR-mediated mitotic checkpoints are not fully elucidated.

Purpose of the Study:

  • To identify novel molecular players in CHFR-mediated mitotic transitions.
  • To elucidate the roles of TOPK/PBK and PTEN in the CHFR mitotic checkpoint pathway.

Main Methods:

  • Ubiquitination assays to determine CHFR's effect on TOPK.
  • Phosphorylation assays to assess TOPK's impact on PTEN.
  • Analysis of G2/M phase progression.

Main Results:

  • CHFR ubiquitinates and regulates the levels of TOPK/PBK, a serine/threonine kinase.
  • TOPK/PBK phosphorylates and inactivates PTEN, a lipid phosphatase.
  • This pathway involving CHFR, TOPK, and PTEN is crucial for proper G2/M progression and mitotic checkpoint function.

Conclusions:

  • TOPK/PBK and PTEN are identified as new key components in the CHFR-mediated mitotic checkpoint.
  • The findings provide a deeper understanding of the molecular regulation of mitosis and genomic stability.
Keywords:
AktChfrE3 ligaseMitosisPTENTOPK

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