Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diagnosing breast masses in BI-RADS 4: construction and interpretation of an intralesional and perilesional radiomics nomogram based on digital breast tomosynthesis.

BMC medical imaging·2026

Same author

Enabling Closed-Loop Recycling of Carbon Fiber-Reinforced Composites: A Dynamic Network Strategy Based on Cardanol-Derived Amines and Lignin-Derived Carbonates.

ACS applied materials & interfaces·2026

Same author

Au@CeO<sub>2</sub> nanozyme-based targeted delivery platform for computed tomography-guided synergistic antioxidant/mild photothermal therapy of inflammatory bowel disease.

Journal of materials chemistry. B·2026

Same author

Protective effects of Demethylfuropinnarin on porcine pre-implantation embryos under Tunicamycin-induced oxidative stress and endoplasmic reticulum stress during in vitro culture.

Scientific reports·2026

Same author

Development of a ratiometric fluorescence nanosensor for evaluating precision chemotherapy of osteosarcoma through real-time monitoring of caspase-3 activity.

Mikrochimica acta·2025

Same author

Self-incompatibility in monoecious Betula platyphylla involves S-RNase mechanisms.

Plant physiology·2025

Search research articles

Related Experiment Video

Updated: May 8, 2026

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

Transcriptomic analysis of incised leaf-shape determination in birch.

Huaizhi Mu¹, Lin Lin, Guifeng Liu

¹State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; Forestry College, Beihua University, 3999 Binjiang East Road, Jilin 132013, China.

|September 10, 2013

Summary

Investigating Betula pendula leaf shape variation revealed significant transcriptome changes. Altered auxin transport and signaling in

Keywords:

6-benzylamino purine 6BA Auxin Betula pendula COG CTAB DEUs FDR GA3 GO Incised leaf KEGG Kyoto Encyclopedia of Genes and Genomes NGS RPKM Transcriptome WPM cluster of orthologous groups of proteins differential expression of unigenes false discovery rate gene ontology gibberellin A3 hexadecyltrimethylammonium bromide next generation sequencing reads per kb per million reads woody plant medium

More Related Videos

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

Related Experiment Videos

Last Updated: May 8, 2026

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

Area of Science:

Plant biology
Genomics
Molecular genetics

Background:

Leaf shape is crucial for plant identification and understanding genetic variation within species.
Betula pendula exhibits natural variations in leaf morphology, such as the distinct incised leaves of 'Dalecarlica' compared to the ovate leaves of the typical species.

Purpose of the Study:

To investigate the impact of intraspecies leaf-shape variation on the transcriptome of Betula pendula.
To compare gene expression profiles between Betula pendula 'Dalecarlica' and typical Betula pendula.
To identify potential genetic pathways underlying leaf shape differences.

Main Methods:

Generating Betula pendula and B. pendula 'Dalecarlica' saplings via tissue culture.
Comparing leaf morphology between the two groups.
Performing transcriptome analysis to identify differentially expressed genes (unigenes).
Conducting pathway analysis to understand functional implications of gene expression changes.

Main Results:

Significant differences in gene expression were observed between B. pendula 'Dalecarlica' and B. pendula.
Upregulation of 8767 unigenes and downregulation of 8379 unigenes in B. pendula 'Dalecarlica' compared to B. pendula.
Pathway analysis indicated alterations in auxin transport and signal transduction in 'Dalecarlica' potentially contributing to its incised leaf shape.

Conclusions:

Intraspecies variation in Betula pendula leaf shape is associated with substantial transcriptome changes.
Altered auxin pathways may play a key role in the development of distinct leaf morphologies in birch.
This study provides insights into the genetic basis of leaf shape variation and identifies candidate genes for birch genetic engineering.